Journal: Neurobiology of Disease

Article Title: Fibroblast growth factors preserve blood-brain barrier integrity through RhoA inhibition after intracerebral hemorrhage in mice

doi: 10.1016/j.nbd.2012.01.008

Figure Lengend Snippet: Effects of exogenous FGF1 (100ng/kg) and FGF2 (30 and 100ng/kg) administration on Garcia (A) and Cylinder Test Scores (B) at 72 hours after surgery. FGF1 and FGF2, improved sensorimotor functions in mice subjected to collagenase induced ICH. Effects of FGF2 on hemorrhage volume (C), Evans blue extravasation (D) and brain water content (E) at 72 hours after surgery. Data are expressed as mean ± SEM. * p<0.05 compared to sham, # p<0.05 compared to vehicle, & p<0.05 compared to FGF2-30ng. N=6–8 per group. Ipsi-CX: ipsilateral cortex, Cont-CX: contralateral cortex, Ipsi-BG: ipsilateral basal ganglia, Cont-BG: contralateral basal ganglia, Cerebel: cerebellum.

Article Snippet: Recombinant human acid fibroblast growth factor (FGF1), recombinant human basic fibroblast growth factor (FGF2), (R&D Systems, Minneapolis, MN), and ROCK inhibitor Y27632 (Ascent Technology, Inc. Cambridge, MA) were dissolved in sterile PBS.

Techniques:

Journal: Neurobiology of Disease

Article Title: Fibroblast growth factors preserve blood-brain barrier integrity through RhoA inhibition after intracerebral hemorrhage in mice

doi: 10.1016/j.nbd.2012.01.008

Figure Lengend Snippet: Effects of exogenous FGF2 (100ng/kg) administration on Garcia Test Score (A) and brain water content (B) at 72 hours after intrastriatal injection of autologous blood in mice. Data are expressed as mean ± SEM. * p<0.05 compared to sham, # p<0.05 compared to vehicle. N=8 per group. Ipsi-CX: ipsilateral cortex, Cont-CX: contralateral cortex, Ipsi-BG: ipsilateral basal ganglia, Cont-BG: contralateral basal ganglia, Cerebel: cerebellum.

Article Snippet: Recombinant human acid fibroblast growth factor (FGF1), recombinant human basic fibroblast growth factor (FGF2), (R&D Systems, Minneapolis, MN), and ROCK inhibitor Y27632 (Ascent Technology, Inc. Cambridge, MA) were dissolved in sterile PBS.

Techniques: Injection

Journal: Neurobiology of Disease

Article Title: Fibroblast growth factors preserve blood-brain barrier integrity through RhoA inhibition after intracerebral hemorrhage in mice

doi: 10.1016/j.nbd.2012.01.008

Figure Lengend Snippet: Effects of FGFR inhibitor PD173074 (0.1 and 0.5 nmol/kg) and DMSO on FGF2 (100ng/kg) induced attenuation of brain injury at 72 hours after surgery. Evaluation of Garcia (A) and Cylinder Test Scores (B), Evans blue extravasation (C) and brain water content (D) in mice subjected to collagenase induced ICH. Data are expressed as mean ± SEM. * p<0.05 compared to sham, # p<0.05 compared to vehicle, & p<0.05 compared to FGF2-30ng. N=6–8 per group. Ipsi-CX: ipsilateral cortex, Cont-CX: contralateral cortex, Ipsi-BG: ipsilateral basal ganglia, Cont-BG: contralateral basal ganglia, Cerebel: cerebellum.

Article Snippet: Recombinant human acid fibroblast growth factor (FGF1), recombinant human basic fibroblast growth factor (FGF2), (R&D Systems, Minneapolis, MN), and ROCK inhibitor Y27632 (Ascent Technology, Inc. Cambridge, MA) were dissolved in sterile PBS.

Techniques:

Journal: Neurobiology of Disease

Article Title: Fibroblast growth factors preserve blood-brain barrier integrity through RhoA inhibition after intracerebral hemorrhage in mice

doi: 10.1016/j.nbd.2012.01.008

Figure Lengend Snippet: Representative Western blots and densitometric quantification of p-FGFR/β-Actin (A), p-Akt/β-Actin (B), GTP-Rac1/Total-Rac1 (C), GTP-RhoA/Total-RhoA (D), P120-Catenin/VE-Cadherin (E) and β-Catenin/VE-Cadherin (F) at 72 hours after collagenase induced ICH or sham surgery. Data are expressed as mean ± SEM. * p<0.05 compared to sham, # p<0.05 compared to vehicle, & p<0.05 compared to FGF2-30ng. N=6 per group.

Article Snippet: Recombinant human acid fibroblast growth factor (FGF1), recombinant human basic fibroblast growth factor (FGF2), (R&D Systems, Minneapolis, MN), and ROCK inhibitor Y27632 (Ascent Technology, Inc. Cambridge, MA) were dissolved in sterile PBS.

Techniques: Western Blot

Journal: Neurobiology of Disease

Article Title: Fibroblast growth factors preserve blood-brain barrier integrity through RhoA inhibition after intracerebral hemorrhage in mice

doi: 10.1016/j.nbd.2012.01.008

Figure Lengend Snippet: Effects of PI3K inhibitor LY294002 (50nmol/kg) on FGF2 (100ng/kg) induced attenuation of brain injury at 72 hours after surgery. Evaluation of Garcia (A) and Cylinder Test Scores (B), Evans blue extravasation (C) and brain water content (D) in mice subjected to collagenase induced ICH. Data are expressed as mean ± SEM. * p<0.05 compared to sham, # p<0.05 compared to vehicle, & p<0.05 compared to FGF2-30ng. N=6–8 per group. Ipsi-CX: ipsilateral cortex, Cont-CX: contralateral cortex, Ipsi-BG: ipsilateral basal ganglia, Cont-BG: contralateral basal ganglia, Cerebel: cerebellum.

Article Snippet: Recombinant human acid fibroblast growth factor (FGF1), recombinant human basic fibroblast growth factor (FGF2), (R&D Systems, Minneapolis, MN), and ROCK inhibitor Y27632 (Ascent Technology, Inc. Cambridge, MA) were dissolved in sterile PBS.

Techniques:

Journal: Neurobiology of Disease

Article Title: Fibroblast growth factors preserve blood-brain barrier integrity through RhoA inhibition after intracerebral hemorrhage in mice

doi: 10.1016/j.nbd.2012.01.008

Figure Lengend Snippet: Representative Western blots and densitometric quantification of p-Akt/β-Actin (A), GTP-Rac1/Total-Rac1 (B), GTP-RhoA/Total-RhoA (C), P120-Catenin/VE-Cadherin (D) and β-Catenin/VE-Cadherin (E) at 72 hours after collagenase induced ICH or sham surgery. Data are expressed as mean ± SEM. * p<0.05 compared to sham, # p<0.05 compared to vehicle, & p<0.05 compared to FGF2-30ng. N=6 per group.

Article Snippet: Recombinant human acid fibroblast growth factor (FGF1), recombinant human basic fibroblast growth factor (FGF2), (R&D Systems, Minneapolis, MN), and ROCK inhibitor Y27632 (Ascent Technology, Inc. Cambridge, MA) were dissolved in sterile PBS.

Techniques: Western Blot

Journal: Neurobiology of Disease

Article Title: Fibroblast growth factors preserve blood-brain barrier integrity through RhoA inhibition after intracerebral hemorrhage in mice

doi: 10.1016/j.nbd.2012.01.008

Figure Lengend Snippet: Proposed mechanism underlying FGF-mediated inhibition of RhoA after ICH. Thrombin, produced through activation of the coagulation cascade, binds on endothelial PAR-1, leading to activation of RhoA and ROCK. The latter induces phosphorylation and disintegration of adheres junctions proteins, thus facilitating the development of vasogenic brain edema after ICH. FGFs suppress RhoA via FGFR-induced activation of the PI3K-Akt-Rac1 signaling pathway. Co-administrations of FGFR inhibitor PD173074 or PI3K inhibitor LY294002, combined with FGF2 were utilized to study the suggested signaling pathway. Y27632 was additionally administered to confirm that ROCK inhibition confers neuroprotection after ICH. FGFs: Fibroblast growth factors, FGFR: Fibroblast growth factor receptor, ICH: intracerebral hemorrhage, PAR-1: Protease-activated receptor-1.

Article Snippet: Recombinant human acid fibroblast growth factor (FGF1), recombinant human basic fibroblast growth factor (FGF2), (R&D Systems, Minneapolis, MN), and ROCK inhibitor Y27632 (Ascent Technology, Inc. Cambridge, MA) were dissolved in sterile PBS.

Techniques: Inhibition, Produced, Activation Assay, Coagulation

Journal: eLife

Article Title: 16p11.2 microdeletion imparts transcriptional alterations in human iPSC-derived models of early neural development

doi: 10.7554/eLife.58178

Figure Lengend Snippet:

Article Snippet: Peptides, Recombinant Proteins , Human Recombinant FGF2 , R and D Systems , Cat. No. 233-FB , .

Techniques: Recombinant, DNA Purification, Purification, Reverse Transcription, Transgenic Assay, Sequencing, Software, Genome Wide, Functional Assay

Journal: Cell reports

Article Title: Self-organization of polarized cerebellar tissue in 3D culture of human pluripotent stem cells.

doi: 10.1016/j.celrep.2014.12.051

Figure Lengend Snippet: Figure 1. Selective Generation of Cerebellar-Plate-like NE in Self-Organizing hESC Culture (A) Procedure for CP-specific SFEBq culture of hESCs. (B) qPCR analysis for region-specific genes in hESC aggregate cultured for 21 days with or without FGF2. (C and D) Immunostaining for OTX2, GBX2, and N-cadherin of hESC aggregates (day 21) cultured with or without FGF2.

Article Snippet: Defining the day on which the SFEBq culture was started as day 0, recombinant human FGF2 (R&D Systems; 50 ng/ml) was added to culture on day 2.

Techniques: Cell Culture, Immunostaining

Journal: Cell reports

Article Title: Self-organization of polarized cerebellar tissue in 3D culture of human pluripotent stem cells.

doi: 10.1016/j.celrep.2014.12.051

Figure Lengend Snippet: Figure 5. Self-Formation of Cerebellar NE with RL-like Structure in FGF2+FGF19+SDF1-Treated hESC Aggregate (A–D) Self-formation of NE structure in hESC aggregate. Immunostaining for PKCz(aPKC) and N-cadherin. (E) Procedure of FGF2 (50 ng/ml), FGF19 (100 ng/ml), and SDF1 (300 ng/ml) treatments.

Article Snippet: Defining the day on which the SFEBq culture was started as day 0, recombinant human FGF2 (R&D Systems; 50 ng/ml) was added to culture on day 2.

Techniques: Immunostaining